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991.
992.
993.
高效液相色谱法测定人血浆中翁丹西隆浓度 总被引:1,自引:0,他引:1
建立了翁丹西隆的高效液相色谱测定法。血浆样品用醋酸乙酯萃取,以ZorbaxSilica为固定相,60%0.025mol/L乙酸钠缓冲液(pH4.2)、40%乙腈为流动相,紫外310nm处测定。采用外标法峰高定量。最低检出浓度达1ng/ml,线性范围1.0~40.0ng/ml(r=0.9996),平均回收率为(95.22±3.58)%(RSD3.76%)。用本法测定人血浆中翁丹西隆浓度具有快速、简便、专一性和灵敏度高的优点。 相似文献
994.
应用ELISA测定了54例类风湿性关节炎患者、99例非类风湿性关节炎患者以及100例正常人的血清Ⅱ型胶原抗体,结果阳性率分别为90.7%、0、0.54例类风湿性关节炎患者中有7例血清类风湿因子为阴性而Ⅱ型胶原抗体为阳性,且病程均在半年之内。结果表明:Ⅱ型胶原抗体的检测对类风湿性关节炎具有特异性诊断和早期诊断的临床意义。 相似文献
995.
996.
Abstract The study of animal lectins and glycoconjugates has become an important area of research in biomedical sciences, as these molecules are believed to play important roles in a variety of biological processes. This report describes a study of the expression of an animal lectin, IgE-binding protein (?BP), also known as Mac-2 and CBP35, in human skin. We have analyzed cultured human keratinocytes as well as normal human skin and a number of epidermal neoplasms, by immunoblotting. immuno-fluorescence and immunohistochemistry. We showed that ?BP is expressed in human keratinocytes, hair follicles, sebaceous and sweat glands. We found that cBP expression retains in various epidermal neoplasms, including basal cell carcinoma, squamous cell carcinoma and keratoacan-thoma, although the level of expression appears to be reduced as compared to normal epidermis. The immunohistochemical analysis also suggests that the level of ?BP expression appears to be dependent on the degree of cellular differentiation of keratinocytes. 相似文献
997.
采用电磁血流仪和血气分析等技术,研究普鲁卡因静脉复合麻醉对犬体循环血流动力学及肝脏血流和氧耗影响。 1%普鲁卡因复合液以 1.0mg· kg-1/min速度静滴时,随麻醉时间延长,血浆普鲁卡因浓度逐渐升高,60分钟达56.9±8.7mg/L。体循环各项血流动力学参数趋于稳定,仅在60分钟产生明显的体循环抑制效应。心率和平均动脉压分别降低27%、25%,心指数由3.52±0.21L·min-1/m2降至 3. 08±0.12L· min-1/m2。肝总血流量和氧耗与体循环动力学呈一致性变化。因此,肝脏疾患及肝手术的患者,只要控制血中普鲁卡因浓度不过高,仍可安全实施普鲁卡因静脉复合麻醉。 相似文献
998.
P Liu W G Williams G Webb J Butany P R McLaughlin 《The Canadian journal of cardiology》1992,8(2):200-203
Blalock-Taussig shunts (subclavian to pulmonary anastomoses) have remained the most effective palliation in cyanotic heart disease. Late complications are rare but can be devastating. The case of a 26-year-old female with tetralogy and an original Blalock shunt constructed at age four years is reported. Despite subsequent primary repair of the tetralogy and presumed ligation of the Blalock shunt, the patient succumbed to a fatal hemorrhage due to esophageal-arterial fistula involving the Blalock shunt. The circumstances leading to this dramatic outcome are outlined and discussed. It is important for cardiologists caring for congenital heart disease patients to be aware of the late complications of congenital heart surgery, and carry out the proper follow-up investigations. 相似文献
999.
50例SLE患者有肺胸膜病变,其中女性47例(94%),男性3例(6%),小于40岁者41例(82%)。50例中表现为胸膜炎和(或)胸腔积液26例,狼疮肺炎15例,弥漫性肺间质纤维化5例,肺不张3例,肺纹理增多17例。经激素治疗随SLE症状改善,肺胸膜病变好转。 相似文献
1000.
The SSEPs in 115 patients with head trauma were analysed by NT+ W value, da, db and b/a methods. The results revealed that the abnormal rates of the SSEPs were 94.8%. The pathological bases resulting in it were the direct and/or indirect lesion on the somatosensory pathway. The SSEPs recorded six months after the trauma indicated that the electric activities of neurons in some of the patients were still abnormal although they recovered well. The patients complicated with traumatic lesion of acoustic nerve, blood clots in external ear canal and pre-existing hearing loss had abnormal BAEPs that could not be used in diagnosing brainstem lesion. The patients without these complications in whom BAEPs were abnormal had an unfavourable outcome. The SSEPs and BAEPs simultaneously recorded could aid localization of the injury in the brain and enable discovery of the fact that the rigidity after decortication or decerebration is a result of severe injury of the cerebral hemisphere. 相似文献